BAIT

SPB4

L000001991, YFL002C
Putative ATP-dependent RNA helicase; nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients
GO Process (3)
GO Function (1)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

RRP3

RNA-dependent ATPase RRP3, L000004225, YHR065C
Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity
GO Process (1)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Dynamics of the putative RNA helicase Spb4 during ribosome assembly in Saccharomyces cerevisiae.

Garcia-Gomez JJ, Lebaron S, Froment C, Monsarrat B, Henry Y, de la Cruz J

Spb4 is a putative ATP-dependent RNA helicase required for proper processing of 27SB pre-rRNAs and therefore for 60S ribosomal subunit biogenesis. To define the timing of association of this protein with pre-ribosomal particles, we have studied the composition of complexes that copurify with TAP-tagged Spb4. These complexes contain mainly the 27SB pre-rRNAs and about 50 ribosome biogenesis proteins, primarily components ... [more]

Unknown Aug. 08, 2011; 0(0); [Pubmed: 21825077]

Throughput

  • High Throughput

Additional Notes

  • factors associated with Spb4-TAP or dominant negative Spb4(R360A)-TAP

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SPB4 RRP3
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2215BioGRID
1930687

Curated By

  • BioGRID