An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Mindbomb 1, an E3 ubiquitin ligase, forms a complex with RYK to activate Wnt/{beta}-catenin signaling.

Berndt JD, Aoyagi A, Yang P, Anastas JN, Tang L, Moon RT

Receptor-like tyrosine kinase (RYK) functions as a transmembrane receptor for the Wnt family of secreted protein ligands. Although RYK undergoes endocytosis in response to Wnt, the mechanisms that regulate its internalization and concomitant activation of Wnt signaling are unknown. We discovered that RYK both physically and functionally interacts with the E3 ubiquitin ligase Mindbomb 1 (MIB1). Overexpression of MIB1 promotes ... [more]

J. Cell Biol. Sep. 05, 2011; 194(5);737-50 [Pubmed: 21875946]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

RYK

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein tyrosine kinase activity [IDA]transmembrane receptor protein tyrosine kinase activity [TAS]transmembrane signaling receptor activity [IDA]

Gene Ontology Cellular Component

RHOT2