BAIT

CDC34

DNA6, UBC3, SCF E2 ubiquitin-protein ligase catalytic subunit CDC34, L000000271, YDR054C
Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

CHS5

CAL3, L000000333, YLR330W
Component of the exomer complex; exomer also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane; Chs5p is the only protein with a BRCT domain that is not localized to the nucleus
Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

New insight into the role of the Cdc34 ubiquitin-conjugating enzyme in cell cycle regulation via Ace2 and Sic1.

Cocklin R, Heyen J, Larry T, Tyers M, Goebl M

The Cdc34 ubiquitin-conjugating enzyme plays a central role in progression of the cell cycle. Through analysis of the phenotype of a mutant missing a highly conserved sequence motif within the catalytic domain of Cdc34, we discovered previously unrecognized levels of regulation of the Ace2 transcription factor and the cyclin-dependent protein kinase inhibitor Sic1. In cells carrying the Cdc34(tm) mutation, which ... [more]

Genetics Mar. 01, 2011; 187(3);701-15 [Pubmed: 21196523]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: colony size (APO:0000063)
  • phenotype: vegetative growth (APO:0000106)

Additional Notes

  • High Throughput: A Synthetic Genetic Array (SGA) screen was carried out using a CDC34tm neomorphic mutant as the query strain.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CHS5 CDC34
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1337BioGRID
2058500

Curated By

  • BioGRID