BAIT
CDC34
DNA6, UBC3, SCF E2 ubiquitin-protein ligase catalytic subunit CDC34, L000000271, YDR054C
Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress
GO Process (6)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [TAS]
- G2/M transition of mitotic cell cycle [IGI]
- SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [IDA]
- protein autoubiquitination [IDA, IMP]
- protein polyubiquitination [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IDA]
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
PREY
DYN2
SLC1, dynein light chain, L000003064, YDR424C
Cytoplasmic light chain dynein, microtubule motor protein; required for intracellular transport and cell division; involved in mitotic spindle positioning; forms complex with dynein intermediate chain Pac11p that promotes Dyn1p homodimerization, potentiates motor processivity; Dyn2p-Pac11p complex important for interaction of dynein motor complex with dynactin complex; acts as molecular glue to dimerize, stabilize Nup82-Nsp1-Nup159 complex module of cytoplasmic pore filaments
GO Process (4)
GO Function (1)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
New insight into the role of the Cdc34 ubiquitin-conjugating enzyme in cell cycle regulation via Ace2 and Sic1.
The Cdc34 ubiquitin-conjugating enzyme plays a central role in progression of the cell cycle. Through analysis of the phenotype of a mutant missing a highly conserved sequence motif within the catalytic domain of Cdc34, we discovered previously unrecognized levels of regulation of the Ace2 transcription factor and the cyclin-dependent protein kinase inhibitor Sic1. In cells carrying the Cdc34(tm) mutation, which ... [more]
Genetics Mar. 01, 2011; 187(3);701-15 [Pubmed: 21196523]
Throughput
- High Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
- phenotype: colony size (APO:0000063)
Additional Notes
- High Throughput: A Synthetic Genetic Array (SGA) screen was carried out using a CDC34tm neomorphic mutant as the query strain.
Curated By
- BioGRID