BAIT

PPP4C

PP4, PP4C, PPH3, PPP4, PPX
protein phosphatase 4, catalytic subunit
Homo sapiens
PREY

ATAD3A

RP5-832C2.1
ATPase family, AAA domain containing 3A
GO Process (2)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

An integrated workflow for charting the human interaction proteome: insights into the PP2A system.

Glatter T, Wepf A, Aebersold R, Gstaiger M

Protein complexes represent major functional units for the execution of biological processes. Systematic affinity purification coupled with mass spectrometry (AP-MS) yielded a wealth of information on the compendium of protein complexes expressed in Saccharomyces cerevisiae. However, global AP-MS analysis of human protein complexes is hampered by the low throughput, sensitivity and data robustness of existing procedures, which limit its application ... [more]

Mol. Syst. Biol. Jan. 22, 2009; 5(0);237 [Pubmed: 19156129]

Throughput

  • Low Throughput

Curated By

  • BioGRID