An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

L3MBTL1 polycomb protein, a candidate tumor suppressor in del(20q12) myeloid disorders, is essential for genome stability.

Gurvich N, Perna F, Farina A, Voza F, Menendez S, Hurwitz J, Nimer SD

The l3mbtl1 gene is located on the long arm of chromosome 20 (q12), within a region commonly deleted in several myeloid malignancies. L3MBTL1 is a human homolog of the Drosophila polycomb L(3)MBT tumor suppressor protein and thus a candidate tumor suppressor in del(20q12) myeloid disorders. We used the loss-of-function approach to explore the possible tumor suppressive mechanism of L3MBTL1 and ... [more]

Proc. Natl. Acad. Sci. U.S.A. Dec. 28, 2010; 107(52);22552-7 [Pubmed: 21149733]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

L3MBTL1

Gene Ontology Biological Process

Gene Ontology Molecular Function

SAM domain binding [IPI]chromatin binding [IDA]histone binding [IPI]identical protein binding [IPI]methylated histone binding [IDA, IPI]nucleosomal histone binding [IDA]nucleosome binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

MCM3