BAIT

CRYGE

Cryg-2, Cryg-6, DGcry-2, Elo
crystallin, gamma E
GO Process (2)
GO Function (0)
GO Component (0)

Gene Ontology Biological Process

Mus musculus
PREY

MYH3

MyHC-emb, Myhs-e, Myhse, RP23-294E23.3
myosin, heavy polypeptide 3, skeletal muscle, embryonic
GO Process (2)
GO Function (2)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Ozz-E3 ubiquitin ligase targets sarcomeric embryonic myosin heavy chain during muscle development.

Campos Y, Qiu X, Zanoteli E, Moshiach S, Vergani N, Bongiovanni A, Harris AJ, d'Azzo A

Muscle contractile proteins are expressed as a series of developmental isoforms that are in constant dynamic remodeling during embryogenesis, but how obsolete molecules are recognized and removed is not known. Ozz is a developmentally regulated protein that functions as the adaptor component of a RING-type ubiquitin ligase complex specific to striated muscle. Ozz(-/-) mutants exhibit defects in myofibrillogenesis and myofiber ... [more]

PLoS ONE Mar. 31, 2010; 5(3);e9866 [Pubmed: 20352047]

Throughput

  • Low Throughput

Curated By

  • BioGRID