BAIT

ICT1

DS-1, DS1, MRP-L58
immature colon carcinoma transcript 1
GO Process (1)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

SLC25A18

GC2
solute carrier family 25 (glutamate carrier), member 18
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A functional peptidyl-tRNA hydrolase, ICT1, has been recruited into the human mitochondrial ribosome.

Richter R, Rorbach J, Pajak A, Smith PM, Wessels HJ, Huynen MA, Smeitink JA, Lightowlers RN, Chrzanowska-Lightowlers ZM

Bioinformatic analysis classifies the human protein encoded by immature colon carcinoma transcript-1 (ICT1) as one of a family of four putative mitochondrial translation release factors. However, this has not been supported by any experimental evidence. As only a single member of this family, mtRF1a, is required to terminate the synthesis of all 13 mitochondrially encoded polypeptides, the true physiological function ... [more]

EMBO J. Mar. 17, 2010; 29(6);1116-25 [Pubmed: 20186120]

Throughput

  • High Throughput

Ontology Terms

  • hek-293 cell (BTO:0000007)

Additional Notes

  • Exogenous expression of bait

Curated By

  • BioGRID