BAIT

MET4

L000001079, YNL103W
Leucine-zipper transcriptional activator; responsible for regulation of sulfur amino acid pathway; requires different combinations of auxiliary factors Cbf1p, Met28p, Met31p and Met32p; feedforward loop exists in the regulation of genes controlled by Met4p and Met32p; can be ubiquitinated by ubiquitin ligase SCF-Met30p, is either degraded or maintained in an inactive state; regulates degradation of its own DNA-binding cofactors by targeting them to SCF-Met30p
Saccharomyces cerevisiae (S288c)
PREY

SAM2

ETH2, methionine adenosyltransferase SAM2, L000001799, YDR502C
S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM2 has a paralog, SAM1, that arose from the whole genome duplication
GO Process (2)
GO Function (1)
GO Component (0)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Saccharomyces cerevisiae (S288c)

Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

Coordinated regulation of sulfur and phospholipid metabolism reflects the importance of methylation in the growth of yeast.

Hickman MJ, Petti AA, Ho-Shing O, Silverman S, McIsaac RS, Lee TA, Botstein D

A yeast strain lacking Met4p, the primary transcriptional regulator of the sulfur assimilation pathway, cannot synthesize methionine. We were surprised to find that this apparently simple auxotroph doesn't grow well in rich media containing excess methionine, forming small colonies on YPD plates. Faster-growing large colonies were abundant when overnight cultures were plated, suggesting that spontaneous suppressors of the growth defect ... [more]

Unknown Sep. 07, 2011; 0(0); [Pubmed: 21900497]

Throughput

  • Low Throughput

Ontology Terms

  • vegetative growth (APO:0000106)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MET4 SAM2
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
162038

Curated By

  • BioGRID