BAIT
CD4
CD4mut
CD4 molecule
GO Process (19)
GO Function (11)
GO Component (5)
Gene Ontology Biological Process
- T cell costimulation [TAS]
- T cell differentiation [IDA]
- T cell receptor signaling pathway [TAS]
- T cell selection [IDA]
- cell surface receptor signaling pathway [TAS]
- entry into host cell [TAS]
- enzyme linked receptor protein signaling pathway [TAS]
- immune response [NAS]
- induction by virus of host cell-cell fusion [IDA]
- innate immune response [TAS]
- maintenance of protein location in cell [IDA]
- positive regulation of interleukin-2 biosynthetic process [NAS]
- positive regulation of protein kinase activity [IDA]
- protein palmitoleylation [IDA]
- regulation of T cell activation [IDA]
- regulation of defense response to virus by virus [TAS]
- signal transduction [TAS]
- transmembrane receptor protein tyrosine kinase signaling pathway [NAS]
- viral process [TAS]
Gene Ontology Molecular Function- MHC class II protein binding [NAS]
- coreceptor activity [NAS]
- enzyme binding [IPI]
- extracellular matrix structural constituent [NAS]
- glycoprotein binding [IPI]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- protein kinase binding [IPI]
- receptor activity [TAS]
- transmembrane signaling receptor activity [TAS]
- zinc ion binding [IDA]
- MHC class II protein binding [NAS]
- coreceptor activity [NAS]
- enzyme binding [IPI]
- extracellular matrix structural constituent [NAS]
- glycoprotein binding [IPI]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- protein kinase binding [IPI]
- receptor activity [TAS]
- transmembrane signaling receptor activity [TAS]
- zinc ion binding [IDA]
Gene Ontology Cellular Component
Homo sapiens
PREY
NPM1
B23, NPM
nucleophosmin (nucleolar phosphoprotein B23, numatrin)
GO Process (23)
GO Function (14)
GO Component (10)
Gene Ontology Biological Process
- CENP-A containing nucleosome assembly [TAS]
- DNA repair [IDA]
- cell aging [IMP, ISS]
- centrosome cycle [IMP, ISS]
- intracellular protein transport [TAS]
- negative regulation of apoptotic process [IDA, NAS]
- negative regulation of cell proliferation [IMP, ISS]
- negative regulation of centrosome duplication [IMP]
- negative regulation of protein kinase activity by regulation of protein phosphorylation [IDA]
- nucleocytoplasmic transport [IDA, TAS]
- nucleosome assembly [IDA, TAS]
- positive regulation of NF-kappaB transcription factor activity [IMP]
- positive regulation of translation [IDA]
- protein localization [IDA]
- protein oligomerization [IDA]
- regulation of centriole replication [IMP]
- regulation of eIF2 alpha phosphorylation by dsRNA [IDA]
- regulation of endodeoxyribonuclease activity [IDA]
- regulation of endoribonuclease activity [IDA]
- response to stress [IMP]
- ribosome assembly [TAS]
- signal transduction [NAS]
- viral process [TAS]
Gene Ontology Molecular Function- NF-kappaB binding [IDA, ISS]
- RNA binding [IDA]
- Tat protein binding [IDA]
- histone binding [IDA]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- protein heterodimerization activity [IMP]
- protein homodimerization activity [IDA]
- protein kinase binding [IPI]
- protein kinase inhibitor activity [IDA]
- ribosomal large subunit binding [IDA]
- ribosomal small subunit binding [IDA]
- transcription coactivator activity [IDA]
- unfolded protein binding [IDA, ISS]
- NF-kappaB binding [IDA, ISS]
- RNA binding [IDA]
- Tat protein binding [IDA]
- histone binding [IDA]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- protein heterodimerization activity [IMP]
- protein homodimerization activity [IDA]
- protein kinase binding [IPI]
- protein kinase inhibitor activity [IDA]
- ribosomal large subunit binding [IDA]
- ribosomal small subunit binding [IDA]
- transcription coactivator activity [IDA]
- unfolded protein binding [IDA, ISS]
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Analysis of proteins copurifying with the CD4/lck complex using one-dimensional polyacrylamide gel electrophoresis and mass spectrometry: comparison with affinity-tag based protein detection and evaluation of different solubilization methods.
Mass spectrometry-based identification of the components of affinity purified protein complexes after polyacrylamide gel electrophoresis (PAGE) and in-gel digest has become very popular for the detection of novel protein interactions. As an alternative, the entire protein complex can be subjected to proteolytic cleavage followed by chromatographic separation of the peptides. Based on our earlier report of a method using affinity ... [more]
J. Am. Soc. Mass Spectrom. Apr. 01, 2004; 15(4);558-67 [Pubmed: 15047060]
Throughput
- High Throughput
Ontology Terms
- cell line: lymphoblastoid cell line (BTO:0000773)
Curated By
- BioGRID