PREY

SRC

AW259666, pp60c-src, RP23-169M4.1
Rous sarcoma oncogene
GO Process (29)
GO Function (9)
GO Component (6)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Development and validation of a method for profiling post-translational modification activities using protein microarrays.

Del Rincon SV, Rogers J, Widschwendter M, Sun D, Sieburg HB, Spruck C

Post-translational modifications (PTMs) impact on the stability, cellular location, and function of a protein thereby achieving a greater functional diversity of the proteome. To fully appreciate how PTMs modulate signaling networks, proteome-wide studies are necessary. However, the evaluation of PTMs on a proteome-wide scale has proven to be technically difficult. To facilitate these analyses we have developed a protein microarray-based ... [more]

PLoS ONE Jul. 03, 2010; 5(6);e11332 [Pubmed: 20596523]

Throughput

  • Low Throughput

Curated By

  • BioGRID