Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Nucleolar localization of DGCR8 and identification of eleven DGCR8-associated proteins.

Shiohama A, Sasaki T, Noda S, Minoshima S, Shimizu N

We identified 11 proteins that are associated with DGCR8 by immunoprecipitation assay and mass spectrometry. These proteins included Nucleolin, ILF3 and others, most of which appeared to be involved in the RNA processing or RNA transportation. We detected at least four kinds of protein complex, such as DROSHA/DGCR8, DGCR8/Nucleolin, DGCR8/ILF3 and ILF3/XPO5, by co-immunoprecipitation. The complex formation of DGCR8 with ... [more]

Exp. Cell Res. Dec. 10, 2007; 313(20);4196-207 [Pubmed: 17765891]

Throughput

  • Low Throughput

Curated By

  • BioGRID