An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

In mouse brain profilin I and profilin II associate with regulators of the endocytic pathway and actin assembly.

Witke W, Podtelejnikov AV, Di Nardo A, Sutherland JD, Gurniak CB, Dotti C, Mann M

Profilins are thought to be essential for regulation of actin assembly. However, the functions of profilins in mammalian tissues are not well understood. In mice profilin I is expressed ubiquitously while profilin II is expressed at high levels only in brain. In extracts from mouse brain, profilin I and profilin II can form complexes with regulators of endocytosis, synaptic vesicle ... [more]

EMBO J. Feb. 16, 1998; 17(4);967-76 [Pubmed: 9463375]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

DNM1

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [ISO]nitric-oxide synthase binding [ISO]poly(A) RNA binding [ISO]protein C-terminus binding [ISO]protein binding [IPI]protein complex binding [ISO]protein dimerization activity [IDA]

Gene Ontology Cellular Component