TJP1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- adherens junction [IDA]
- apical junction complex [ISO]
- apical part of cell [IDA, ISO]
- apical plasma membrane [IDA]
- apicolateral plasma membrane [IDA]
- basolateral plasma membrane [ISO]
- cell junction [IDA, ISO]
- cell surface [ISO]
- cell-cell adherens junction [IDA]
- cell-cell junction [IDA]
- cytoplasm [IDA, ISO]
- intercalated disc [IDA]
- intercellular canaliculus [IDA]
- membrane [IDA]
- nucleus [IDA]
- plasma membrane [IDA, ISO]
- tight junction [IDA, ISO]
ACTN4
Gene Ontology Biological Process
- actin filament bundle assembly [IDA]
- negative regulation of cellular component movement [IDA]
- positive regulation of cellular component movement [IDA, IMP, ISO]
- positive regulation of pinocytosis [IMP]
- protein localization to tight junction [IMP]
- regulation of apoptotic process [ISO]
- tight junction assembly [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Z disc [IDA]
- cell-cell junction [IDA]
- cortical cytoskeleton [IDA]
- cytoplasm [IDA, ISO]
- extracellular space [ISO]
- extracellular vesicular exosome [ISO]
- focal adhesion [ISO]
- neuron projection [ISO]
- nucleus [ISO]
- perinuclear region of cytoplasm [ISO]
- protein complex [ISO]
- pseudopodium [IDA]
- ribonucleoprotein complex [ISO]
- stress fiber [IDA, ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Interaction of zonula occludens-1 (ZO-1) with alpha-actinin-4: application of functional proteomics for identification of PDZ domain-associated proteins.
The use of recombinant "bait" proteins to capture protein-binding partners, followed by identification of protein interaction networks by mass spectrometry (MS), has gained popularity and widespread acceptance. We have developed an approach using recombinant PDZ protein interaction modules of the membrane-associated guanylate kinase (MAGUK) protein zonula occludens-1 (ZO-1) to pull-down and screen for proteins that interact with these modules via ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID