MCM5
Gene Ontology Biological Process
- DNA replication initiation [IMP]
- chromatin silencing at telomere [IMP]
- double-strand break repair via break-induced replication [IMP]
- establishment of chromatin silencing [IMP]
- negative regulation of chromatin silencing at telomere [IMP]
- nuclear DNA replication [IMP]
- pre-replicative complex assembly involved in nuclear cell cycle DNA replication [IDA, IPI]
- regulation of DNA-dependent DNA replication initiation [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PSF1
Gene Ontology Biological Process
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
Enabling association of the GINS tetramer with the Mcm2-7 complex by phosphorylated Sld2 protein and single-stranded origin DNA.
The Cdc45-Mcm2-7-GINS (CMG) complex is the replication fork helicase in eukaryotes. Sld2 is required for the initiation of DNA replication, and the S-phase cyclin-dependent kinase (S-CDK) phosphorylates Sld2 in vivo. We purified components of the replication initiation machinery and studied their interactions in vitro. We find that unphosphorylated or CDK-phosphorylated Sld2 binds to the Mcm2-7 complex with similar efficiency. Sld2 ... [more]
Throughput
- Low Throughput
Additional Notes
- the tagged MCM2-7 complex binds GINS in vitro
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PSF1 MCM5 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 2 | BioGRID | 3594027 | |
| MCM5 PSF1 | Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps. | Low | - | BioGRID | - | |
| MCM5 PSF1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.5407 | BioGRID | 1944629 | |
| PSF1 MCM5 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.5021 | BioGRID | 1924800 | |
| MCM5 PSF1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - |
Curated By
- BioGRID