SLD3
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
MCM7
Gene Ontology Biological Process
- DNA replication initiation [IGI, IMP]
- DNA strand elongation involved in DNA replication [IGI, IMP]
- DNA unwinding involved in DNA replication [IDA]
- chromatin silencing at silent mating-type cassette [IMP]
- chromatin silencing at telomere [IMP]
- double-strand break repair via break-induced replication [IMP]
- nuclear DNA replication [IMP]
- pre-replicative complex assembly involved in nuclear cell cycle DNA replication [IDA, IPI]
Gene Ontology Molecular Function- ATP binding [IDA]
- ATP-dependent 3'-5' DNA helicase activity [IDA]
- ATP-dependent DNA helicase activity [IDA]
- ATP-dependent four-way junction helicase activity [IDA]
- DNA helicase activity [IDA]
- DNA replication origin binding [IDA]
- chromatin binding [IDA]
- single-stranded DNA binding [IMP]
- single-stranded DNA-dependent ATP-dependent DNA helicase activity [IDA]
- single-stranded DNA-dependent ATPase activity [IDA]
- ATP binding [IDA]
- ATP-dependent 3'-5' DNA helicase activity [IDA]
- ATP-dependent DNA helicase activity [IDA]
- ATP-dependent four-way junction helicase activity [IDA]
- DNA helicase activity [IDA]
- DNA replication origin binding [IDA]
- chromatin binding [IDA]
- single-stranded DNA binding [IMP]
- single-stranded DNA-dependent ATP-dependent DNA helicase activity [IDA]
- single-stranded DNA-dependent ATPase activity [IDA]
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
Enabling association of the GINS tetramer with the Mcm2-7 complex by phosphorylated Sld2 protein and single-stranded origin DNA.
The Cdc45-Mcm2-7-GINS (CMG) complex is the replication fork helicase in eukaryotes. Sld2 is required for the initiation of DNA replication, and the S-phase cyclin-dependent kinase (S-CDK) phosphorylates Sld2 in vivo. We purified components of the replication initiation machinery and studied their interactions in vitro. We find that unphosphorylated or CDK-phosphorylated Sld2 binds to the Mcm2-7 complex with similar efficiency. Sld2 ... [more]
Throughput
- Low Throughput
Additional Notes
- the tagged MCM2-7 complex binds Sld3 in vitro
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SLD3 MCM7 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | Low | - | BioGRID | - | |
| SLD3 MCM7 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.5732 | BioGRID | 1933130 | |
| MCM7 SLD3 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.3399 | BioGRID | 1921622 | |
| SLD3 MCM7 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| SLD3 MCM7 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| MCM7 SLD3 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| SLD3 MCM7 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - |
Curated By
- BioGRID