Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

HSP27 and HSP70 interact with CD10 in C4-2 prostate cancer cells.

Dall'Era MA, Oudes A, Martin DB, Liu AY

CD10 is an approximately 100 kDa transmembrane metallo-endopeptidase. CD10 is strongly expressed by normal prostate epithelium. While only 30% of primary prostate tumors express CD10, it is strongly expressed by most lymph node metastases. The function of CD10 and the interaction between CD10 and other cellular proteins in prostate cancer (CaP) is not well defined. Cellular context may ultimately determine ... [more]

Prostate May. 15, 2007; 67(7);714-21 [Pubmed: 17342744]

Throughput

  • High Throughput

Ontology Terms

  • disease: prostate cancer (DOID:10283)
  • cell line: lncap-c42b4 cell (BTO:0002998)

Curated By

  • BioGRID