BAIT

HIST2H2AC

H2A, H2A-GL101, H2A/q, H2AFQ
histone cluster 2, H2ac
GO Process (0)
GO Function (0)
GO Component (2)

Gene Ontology Cellular Component

Homo sapiens
PREY

TAF15

Npl3, RBP56, TAF2N, TAFII68
TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
GO Process (1)
GO Function (2)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

A histone octamer-like structure within TFIID.

Hoffmann A, Chiang CM, Oelgeschlaeger T, Xie X, Burley SK, Nakatani Y, Roeder RG

The general transcription factor TFIID nucleates initiation complex formation through direct core promoter binding, commits promoters within chromatin to transcription, and mediates the action of transcriptional activators, a phenomenon that may correlate with enhanced TFIID recruitment or conformational changes in TFIID-promoter complexes. Molecular studies of the multiprotein TFIID complex have identified a primary TATA binding subunit (TBP), TBP-associated factors (TAFs) ... [more]

Nature Mar. 28, 1996; 380(6572);356-9 [Pubmed: 8598932]

Throughput

  • Low Throughput

Curated By

  • BioGRID