NCOA3
Gene Ontology Biological Process
- androgen receptor signaling pathway [NAS]
- cellular response to hormone stimulus [IBA]
- histone acetylation [IDA]
- intracellular receptor signaling pathway [IBA]
- positive regulation of gene expression [IMP]
- positive regulation of keratinocyte differentiation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of transcription, DNA-templated [NAS]
- receptor transactivation [TAS]
- regulation of RNA biosynthetic process [IMP]
Gene Ontology Molecular Function- androgen receptor binding [NAS]
- histone acetyltransferase activity [IDA]
- ligand-dependent nuclear receptor binding [IPI]
- ligand-dependent nuclear receptor transcription coactivator activity [IBA]
- nuclear hormone receptor binding [IDA]
- protein N-terminus binding [IPI]
- protein binding [IPI]
- thyroid hormone receptor binding [NAS]
- transcription coactivator activity [IDA, IMP, NAS]
- androgen receptor binding [NAS]
- histone acetyltransferase activity [IDA]
- ligand-dependent nuclear receptor binding [IPI]
- ligand-dependent nuclear receptor transcription coactivator activity [IBA]
- nuclear hormone receptor binding [IDA]
- protein N-terminus binding [IPI]
- protein binding [IPI]
- thyroid hormone receptor binding [NAS]
- transcription coactivator activity [IDA, IMP, NAS]
Gene Ontology Cellular Component
RARA
Gene Ontology Biological Process
- apoptotic cell clearance [IMP]
- cellular response to estrogen stimulus [IDA]
- cellular response to retinoic acid [IDA]
- gene expression [TAS]
- intracellular estrogen receptor signaling pathway [IDA]
- negative regulation of granulocyte differentiation [IDA]
- negative regulation of interferon-gamma production [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- negative regulation of tumor necrosis factor production [IDA]
- positive regulation of T-helper 2 cell differentiation [IDA]
- positive regulation of binding [IMP]
- positive regulation of cell cycle [IMP]
- positive regulation of cell proliferation [IMP]
- positive regulation of interleukin-13 production [IDA]
- positive regulation of interleukin-4 production [IDA]
- positive regulation of interleukin-5 production [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of transcription, DNA-templated [IDA, IMP]
- protein phosphorylation [IMP]
- response to estradiol [IDA]
- response to retinoic acid [IMP]
- retinoic acid receptor signaling pathway [IMP]
- signal transduction [IDA]
- transcription initiation from RNA polymerase II promoter [TAS]
Gene Ontology Molecular Function- chromatin DNA binding [IDA]
- enzyme binding [IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein heterodimerization activity [IDA]
- protein kinase A binding [IDA]
- protein kinase B binding [IPI]
- receptor binding [IDA]
- retinoic acid binding [IDA]
- retinoic acid receptor activity [IDA]
- retinoic acid-responsive element binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription coactivator activity [IDA, IMP]
- transcription corepressor activity [IDA]
- transcription factor binding [IPI]
- chromatin DNA binding [IDA]
- enzyme binding [IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein heterodimerization activity [IDA]
- protein kinase A binding [IDA]
- protein kinase B binding [IPI]
- receptor binding [IDA]
- retinoic acid binding [IDA]
- retinoic acid receptor activity [IDA]
- retinoic acid-responsive element binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription coactivator activity [IDA, IMP]
- transcription corepressor activity [IDA]
- transcription factor binding [IPI]
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Automated yeast two-hybrid screening for nuclear receptor-interacting proteins.
High throughput analysis of protein-protein interactions is an important sector of hypothesis-generating research. Using an improved and automated version of the yeast two-hybrid system, we completed a large interaction screening project with a focus on nuclear receptors and their cofactors. A total of 425 independent yeast two-hybrid cDNA library screens resulted in 6425 potential interacting protein fragments involved in 1613 ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RARA NCOA3 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 657529 | |
| RARA NCOA3 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 658441 | |
| NCOA3 RARA | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| RARA NCOA3 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| RARA NCOA3 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| RARA NCOA3 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| NCOA3 RARA | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID