BAIT

CDK7

CAK1, CDKN7, HCAK, MO15, STK1, p39MO15
cyclin-dependent kinase 7
GO Process (25)
GO Function (7)
GO Component (5)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Isolation and characterization of two human transcription factor IIH (TFIIH)-related complexes: ERCC2/CAK and TFIIH.

Reardon JT, Ge H, Gibbs E, Sancar A, Hurwitz J, Pan ZQ

Transcription factor IIH (TFIIH) is a multisubunit protein complex essential for both the initiation of RNA polymerase class II (pol II)-catalyzed transcription and nucleotide excision repair of DNA. Recent studies have shown that TFIIH copurifies with the cyclin-dependent kinase (cdk)-activating kinase complex (CAK) that includes cdk7, cyclin H, and p36/MAT1. Here we report the isolation of two TFIIH-related complexes: TFIIH* ... [more]

Proc. Natl. Acad. Sci. U.S.A. Jun. 25, 1996; 93(13);6482-7 [Pubmed: 8692841]

Throughput

  • Low Throughput

Curated By

  • BioGRID