BAIT

PRP40

L000003078, YKL012W
U1 snRNP protein involved in splicing; interacts with the branchpoint-binding protein during the formation of the second commitment complex
GO Process (1)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

RPS14A

CRY1, RPL59, ribosomal 40S subunit protein S14A, S11, rp59A, S14A, L000000423, YCR031C
Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)

Protein-peptide

An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.

Publication

Comparative analysis of Saccharomyces cerevisiae WW domains and their interacting proteins.

Hesselberth JR, Miller JP, Golob A, Stajich JE, Michaud GA, Fields S

BACKGROUND: The WW domain is found in a large number of eukaryotic proteins implicated in a variety of cellular processes. WW domains bind proline-rich protein and peptide ligands, but the protein interaction partners of many WW domain-containing proteins in Saccharomyces cerevisiae are largely unknown. RESULTS: We used protein microarray technology to generate a protein interaction map for 12 of the ... [more]

Genome Biol. Apr. 12, 2006; 7(4);R30 [Pubmed: 16606443]

Throughput

  • High Throughput

Additional Notes

  • A protein microarray was probed with the first WW domain from PRP40 (YKL012W_WW1).
  • High Throughput: Each WW-domain was used as a probe for two separate protein microarrays. Only those in which four independent interactions were osbserved were considered high-confidence (i.e. significant signals found for proteins printed in duplicate on two separate microarrays).

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PRP40 RPS14A
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.4271BioGRID
1995492

Curated By

  • BioGRID