RNF31
Gene Ontology Biological Process
- CD40 signaling pathway [IMP]
- T cell receptor signaling pathway [ISO]
- negative regulation of necroptotic process [IGI]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IMP, ISO]
- positive regulation of NF-kappaB transcription factor activity [ISO]
- protein linear polyubiquitination [ISO]
- protein polyubiquitination [ISO]
- protein ubiquitination [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CHUK
Gene Ontology Biological Process
- I-kappaB phosphorylation [ISO, TAS]
- Rho protein signal transduction [ISO]
- cellular response to tumor necrosis factor [ISO]
- lactation [IGI, IMP]
- mammary gland alveolus development [IGI, IMP]
- mammary gland epithelial cell proliferation [IGI, IMP]
- morphogenesis of an epithelial sheet [IMP]
- odontogenesis of dentin-containing tooth [IMP]
- osteoclast differentiation [IGI, IMP]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IGI, IMP]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- protein phosphorylation [IDA, ISO]
- response to lipopolysaccharide [ISO]
- response to muscle stretch [IMP]
- response to organic substance [ISO]
- signal transduction by phosphorylation [TAS]
- striated muscle cell differentiation [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Involvement of linear polyubiquitylation of NEMO in NF-kappaB activation.
Nuclear factor-kappaB (NF-kappaB) is a key transcription factor in inflammatory, anti-apoptotic and immune processes. The ubiquitin pathway is crucial in regulating the NF-kappaB pathway. We have found that the LUBAC ligase complex, composed of the two RING finger proteins HOIL-1L and HOIP, conjugates a head-to-tail-linked linear polyubiquitin chain to substrates. Here, we demonstrate that LUBAC activates the canonical NF-kappaB pathway ... [more]
Throughput
- Low Throughput
Additional Notes
- in the presence of overexpressed nemo
Curated By
- BioGRID