Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The SCO2 protein disulphide isomerase is required for thylakoid biogenesis and interacts with LCHB1 chlorophyl a/b binding proteins which affects chlorophyll biosynthesis in Arabidopsis seedlings.

Tanz S, Kilian J, Johnsson C, Apel K, Small I, Harter K, Wanke D, Pogson B, Albrecht V

The process of chloroplast biogenesis requires a multitude of pathways and processes to establish chloroplast function. In cotyledons of seedlings chloroplasts develop either directly from proplastids (also named eoplasts) or, if germinated in the dark, via etioplasts, whereas in leaves chloroplasts derive from proplastids in the apical meristem and are then multiplied by division. The snowy cotyledon 2, sco2, mutations ... [more]

Unknown Oct. 31, 2011; 0(0); [Pubmed: 22040291]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SCO2 LHB1B2	PCA PCA A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.	Tanz S (2011)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

SCO2

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein disulfide isomerase activity [IDA]protein self-association [IPI]

Gene Ontology Cellular Component

LHB1B2