BAIT

NAB3

HMD1, L000001228, YPL190C

RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with exosome to mediate 3'-end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing; Nrd1-Nab3 pathway appears to have a role in rapid suppression of some genes when cells are shifted to poor growth conditions, indicating role for Nrd1-Nab3 in regulating cellular response to nutrient availability

GO Process (8)

GO Function (3)

GO Component (3)

Gene Ontology Biological Process

CUT catabolic process [IMP]

antisense RNA transcript catabolic process [IMP]

mRNA 3'-end processing [IMP]

nuclear mRNA surveillance [IMP]

snRNA 3'-end processing [IMP]

snoRNA 3'-end processing [IMP]

tRNA 3'-end processing [IMP]

termination of RNA polymerase II transcription, exosome-dependent [IDA]

Gene Ontology Molecular Function

RNA binding [IDA]
mRNA binding [IDA]
transcription regulatory region RNA binding [IDA]

Gene Ontology Cellular Component

Nrd1 complex [IDA]

cytoplasm [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

SNR41

L000003056

C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of small subunit (SSU) rRNA at positions A541 and G1126

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene

Saccharomyces cerevisiae (S288c)

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Transcriptome-Wide Binding Sites for Components of the Saccharomyces cerevisiae Non-Poly(A) Termination Pathway: Nrd1, Nab3, and Sen1.

Creamer TJ, Darby MM, Jamonnak N, Schaughency P, Hao H, Wheelan SJ, Corden JL

RNA polymerase II synthesizes a diverse set of transcripts including both protein-coding and non-coding RNAs. One major difference between these two classes of transcripts is the mechanism of termination. Messenger RNA transcripts terminate downstream of the coding region in a process that is coupled to cleavage and polyadenylation reactions. Non-coding transcripts like Saccharomyces cerevisiae snoRNAs terminate in a process that ... [more]

PLoS Genet. Sep. 01, 2011; 7(10);e1002329 [Pubmed: 22028667]

Throughput

High Throughput

Additional Notes

interaction detected by PAR-CLIP

Curated By

BioGRID

Interaction Summary