BAIT

NAB3

HMD1, L000001228, YPL190C

RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with exosome to mediate 3'-end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing; Nrd1-Nab3 pathway appears to have a role in rapid suppression of some genes when cells are shifted to poor growth conditions, indicating role for Nrd1-Nab3 in regulating cellular response to nutrient availability

GO Process (8)

GO Function (3)

GO Component (3)

Gene Ontology Biological Process

CUT catabolic process [IMP]

antisense RNA transcript catabolic process [IMP]

mRNA 3'-end processing [IMP]

nuclear mRNA surveillance [IMP]

snRNA 3'-end processing [IMP]

snoRNA 3'-end processing [IMP]

tRNA 3'-end processing [IMP]

termination of RNA polymerase II transcription, exosome-dependent [IDA]

Gene Ontology Molecular Function

RNA binding [IDA]
mRNA binding [IDA]
transcription regulatory region RNA binding [IDA]

Gene Ontology Cellular Component

Nrd1 complex [IDA]

cytoplasm [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

SNR191

S000028465

H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at positions U2258 and U2260; encoded within the intron of NOG2/YNR053C

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene

Saccharomyces cerevisiae (S288c)

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Transcriptome-Wide Binding Sites for Components of the Saccharomyces cerevisiae Non-Poly(A) Termination Pathway: Nrd1, Nab3, and Sen1.

Creamer TJ, Darby MM, Jamonnak N, Schaughency P, Hao H, Wheelan SJ, Corden JL

RNA polymerase II synthesizes a diverse set of transcripts including both protein-coding and non-coding RNAs. One major difference between these two classes of transcripts is the mechanism of termination. Messenger RNA transcripts terminate downstream of the coding region in a process that is coupled to cleavage and polyadenylation reactions. Non-coding transcripts like Saccharomyces cerevisiae snoRNAs terminate in a process that ... [more]

PLoS Genet. Sep. 01, 2011; 7(10);e1002329 [Pubmed: 22028667]

Throughput

High Throughput

Additional Notes

interaction detected by PAR-CLIP

Curated By

BioGRID

Interaction Summary