BAIT

RPB2

RPB150, RPO22, SIT2, SOH2, DNA-directed RNA polymerase II core subunit RPB2, B150, L000001588, L000001676, YOR151C
RNA polymerase II second largest subunit B150; part of central core; similar to bacterial beta subunit
GO Process (1)
GO Function (3)
GO Component (3)
Saccharomyces cerevisiae (S288c)
PREY

TMA19

MMI1, RBF18, YKL056C
Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress
GO Process (2)
GO Function (0)
GO Component (4)

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Transcriptome-Wide Binding Sites for Components of the Saccharomyces cerevisiae Non-Poly(A) Termination Pathway: Nrd1, Nab3, and Sen1.

Creamer TJ, Darby MM, Jamonnak N, Schaughency P, Hao H, Wheelan SJ, Corden JL

RNA polymerase II synthesizes a diverse set of transcripts including both protein-coding and non-coding RNAs. One major difference between these two classes of transcripts is the mechanism of termination. Messenger RNA transcripts terminate downstream of the coding region in a process that is coupled to cleavage and polyadenylation reactions. Non-coding transcripts like Saccharomyces cerevisiae snoRNAs terminate in a process that ... [more]

PLoS Genet. Sep. 01, 2011; 7(10);e1002329 [Pubmed: 22028667]

Throughput

  • High Throughput

Additional Notes

  • interaction detected by PAR-CLIP

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TMA19 RPB2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1443BioGRID
2053625

Curated By

  • BioGRID