PREY

TAF3

TAF47, TafII47, L000004154, YPL011C
TFIID subunit (47 kDa); involved in promoter binding and RNA polymerase II transcription initiation
Saccharomyces cerevisiae (S288c)

Synthetic Haploinsufficiency

A genetic interaction is inferred when mutations or deletions in separate genes, at least one of which is hemizygous, cause a minimal phenotype alone but result in lethality when combined in the same cell under a given condition.

Publication

Novel interactions between actin and the proteasome revealed by complex haploinsufficiency.

Haarer B, Aggeli D, Viggiano S, Burke DJ, Amberg DC

Saccharomyces cerevisiae has been a powerful model for uncovering the landscape of binary gene interactions through whole-genome screening. Complex heterozygous interactions are potentially important to human genetic disease as loss-of-function alleles are common in human genomes. We have been using complex haploinsufficiency (CHI) screening with the actin gene to identify genes related to actin function and as a model to ... [more]

PLoS Genet. Sep. 01, 2011; 7(9);e1002288 [Pubmed: 21966278]

Throughput

  • High Throughput|Low Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)

Additional Notes

  • detected in high throughput screen and then hand retested

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TAF3 ACT1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1656BioGRID
1954637

Curated By

  • BioGRID