A genetic interaction is inferred when mutations or deletions in separate genes, at least one of which is hemizygous, cause a minimal phenotype alone but result in lethality when combined in the same cell under a given condition.

Publication

Novel interactions between actin and the proteasome revealed by complex haploinsufficiency.

Haarer B, Aggeli D, Viggiano S, Burke DJ, Amberg DC

Saccharomyces cerevisiae has been a powerful model for uncovering the landscape of binary gene interactions through whole-genome screening. Complex heterozygous interactions are potentially important to human genetic disease as loss-of-function alleles are common in human genomes. We have been using complex haploinsufficiency (CHI) screening with the actin gene to identify genes related to actin function and as a model to ... [more]

PLoS Genet. Sep. 01, 2011; 7(9);e1002288 [Pubmed: 21966278]

Throughput

High Throughput|Low Throughput

Ontology Terms

phenotype: vegetative growth (APO:0000106)

Additional Notes

detected in high throughput screen and then hand retested

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TAF3 ACT1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.1656	BioGRID	1954637

Curated By

BioGRID

Interaction Summary

ACT1

Gene Ontology Biological Process

Gene Ontology Molecular Function

histone acetyltransferase activity [IDA]structural constituent of cytoskeleton [IDA]

Gene Ontology Cellular Component

TAF3