An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

UBXD7 binds multiple ubiquitin ligases and implicates p97 in HIF1alpha turnover.

Alexandru G, Graumann J, Smith GT, Kolawa NJ, Fang R, Deshaies RJ

p97 is an ATP-dependent chaperone that plays an important role in endoplasmic reticulum-associated degradation but whose connections to turnover of soluble proteins remain sparse. Binding of p97 to substrates is mediated by cofactors that contain ubiquitin-binding domains. We employed "network proteomics" to show that p97 assembles with all of the 13 mammalian UBX-domain proteins. The UBX proteins that bind ubiquitin ... [more]

Cell Sep. 05, 2008; 134(5);804-16 [Pubmed: 18775313]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

UBXN1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATPase binding [IPI]K6-linked polyubiquitin binding [IDA]polyubiquitin binding [IDA]protein binding [IPI]ubiquitin binding [IDA]ubiquitin protein ligase binding [IDA]

Gene Ontology Cellular Component

VCPIP1