CLP1
Gene Ontology Biological Process
- RNA splicing [TAS]
- cerebellar cortex development [IMP]
- gene expression [TAS]
- mRNA 3'-end processing [TAS]
- mRNA cleavage [IBA]
- mRNA polyadenylation [IBA]
- mRNA splicing, via spliceosome [TAS]
- siRNA loading onto RISC involved in RNA interference [IDA]
- tRNA splicing, via endonucleolytic cleavage and ligation [IDA, IMP]
- targeting of mRNA for destruction involved in RNA interference [IMP]
- termination of RNA polymerase II transcription [TAS]
- transcription from RNA polymerase II promoter [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CPSF2
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors.
Six different protein factors are required in vitro for 3' end formation of mammalian pre-mRNAs by endonucleolytic cleavage and polyadenylation. Five of the factors have been purified and most of their components cloned, but cleavage factor II(m) (CF II(m)) remained uncharacterized. We have purified CF II(m) from HeLa cell nuclear extract by several chromatographic steps. During purification, CF II(m) activity ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID