Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Characterization of Staufen 1 ribonucleoprotein complexes.

Brendel C, Rehbein M, Kreienkamp HJ, Buck F, Richter D, Kindler S

In Drosophila oocytes and neuroblasts, the double-stranded RNA binding protein Staufen assembles into ribonucleoprotein particles, which mediate cytoplasmic mRNA trafficking and translation. Two different mammalian orthologues also appear to reside in distinct RNA-containing particles. To date, relatively little is known about the molecular composition of Staufen-containing ribonucleoprotein complexes. Here, we have used a novel one-step affinity purification protocol to identify ... [more]

Biochem. J. Dec. 01, 2004; 384(0);239-46 [Pubmed: 15303970]

Throughput

  • Low Throughput

Curated By

  • BioGRID