AXIN1
Gene Ontology Biological Process
- Wnt signaling pathway involved in somitogenesis [IBA]
- Wnt-activated signaling pathway involved in forebrain neuron fate commitment [IBA]
- activation of JUN kinase activity [IBA]
- activation of protein kinase activity [IDA]
- axial mesoderm formation [IBA]
- canonical Wnt signaling pathway [IC]
- canonical Wnt signaling pathway involved in neural plate anterior/posterior pattern formation [IBA]
- cell death [IBA]
- cellular protein complex assembly [IDA]
- cellular response to organic cyclic compound [IBA]
- cytoplasmic microtubule organization [IBA]
- determination of left/right symmetry [IBA]
- dorsal/ventral axis specification [IBA]
- embryonic eye morphogenesis [IBA]
- embryonic skeletal joint morphogenesis [IBA]
- forebrain anterior/posterior pattern specification [IBA]
- muscle cell development [IBA]
- negative regulation of Wnt signaling pathway [IDA]
- negative regulation of canonical Wnt signaling pathway [IBA]
- negative regulation of fat cell differentiation [IBA]
- olfactory placode formation [IBA]
- optic placode formation [IBA]
- positive regulation of GTPase activity [IBA]
- positive regulation of JNK cascade [ISS]
- positive regulation of peptidyl-serine phosphorylation [NAS]
- positive regulation of peptidyl-threonine phosphorylation [NAS]
- positive regulation of protein catabolic process [IC, IDA]
- positive regulation of protein phosphorylation [IDA]
- positive regulation of protein ubiquitination [NAS]
- positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process [ISS]
- positive regulation of transcription, DNA-templated [IMP]
- positive regulation of ubiquitin-protein transferase activity [IMP]
- regulation of catenin import into nucleus [IBA]
Gene Ontology Molecular Function- GTPase activator activity [IBA]
- I-SMAD binding [IPI]
- SMAD binding [IPI]
- armadillo repeat domain binding [IBA, ISS]
- beta-catenin binding [IDA]
- enzyme binding [IPI]
- identical protein binding [ISS]
- protein binding [IPI]
- protein complex scaffold [IDA, IMP]
- protein homodimerization activity [ISS]
- protein kinase binding [IBA, ISS]
- signal transducer activity [IDA]
- ubiquitin protein ligase binding [IPI]
- GTPase activator activity [IBA]
- I-SMAD binding [IPI]
- SMAD binding [IPI]
- armadillo repeat domain binding [IBA, ISS]
- beta-catenin binding [IDA]
- enzyme binding [IPI]
- identical protein binding [ISS]
- protein binding [IPI]
- protein complex scaffold [IDA, IMP]
- protein homodimerization activity [ISS]
- protein kinase binding [IBA, ISS]
- signal transducer activity [IDA]
- ubiquitin protein ligase binding [IPI]
Gene Ontology Cellular Component
- beta-catenin destruction complex [IDA]
- cell cortex [IBA]
- cell periphery [IDA]
- cytoplasm [IDA]
- cytoplasmic membrane-bounded vesicle [IBA]
- cytoplasmic microtubule [IBA]
- cytoplasmic vesicle [ISS]
- cytosol [TAS]
- lateral plasma membrane [IDA]
- nucleus [IDA]
- perinuclear region of cytoplasm [IDA]
- postsynaptic density [IBA]
MYC
Gene Ontology Biological Process
- MAPK cascade [IMP]
- Notch signaling pathway [TAS]
- branching involved in ureteric bud morphogenesis [ISS]
- canonical Wnt signaling pathway [IDA]
- cell cycle arrest [IDA]
- cellular iron ion homeostasis [IDA]
- cellular response to DNA damage stimulus [IDA]
- cellular response to UV [IEP]
- cellular response to drug [IDA]
- chromatin remodeling [IDA]
- chromosome organization [IDA]
- energy reserve metabolic process [NAS]
- fibroblast apoptotic process [TAS]
- gene expression [TAS]
- negative regulation of apoptotic process [ISS]
- negative regulation of cell division [IDA]
- negative regulation of fibroblast proliferation [IDA]
- negative regulation of monocyte differentiation [IMP]
- negative regulation of stress-activated MAPK cascade [ISS]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- oxygen transport [NAS]
- positive regulation of DNA biosynthetic process [IMP]
- positive regulation of cell proliferation [IDA]
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- positive regulation of epithelial cell proliferation [IDA]
- positive regulation of fibroblast proliferation [IDA, IMP]
- positive regulation of mesenchymal cell proliferation [ISS]
- positive regulation of metanephric cap mesenchymal cell proliferation [ISS]
- positive regulation of response to DNA damage stimulus [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP, TAS]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of gene expression [IDA]
- regulation of telomere maintenance [IMP]
- response to drug [IEP]
- response to gamma radiation [IDA]
- response to growth factor [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [TAS]
Gene Ontology Molecular Function- DNA binding [ISS, TAS]
- E-box binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- protein binding [IPI]
- protein complex binding [IDA]
- repressing transcription factor binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI]
- DNA binding [ISS, TAS]
- E-box binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- protein binding [IPI]
- protein complex binding [IDA]
- repressing transcription factor binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The Axin1 scaffold protein promotes formation of a degradation complex for c-Myc.
Expression of the c-Myc proto-oncoprotein is tightly regulated in normal cells. Phosphorylation at two conserved residues, threonine58 (T58) and serine62 (S62), regulates c-Myc protein stability. In cancer cells, c-Myc can become aberrantly stabilized associated with altered T58 and S62 phosphorylation. A complex signalling cascade involving GSK3beta kinase, the Pin1 prolyl isomerase, and the PP2A-B56alpha phosphatase controls phosphorylation at these sites. ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| MYC AXIN1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| AXIN1 MYC | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID