BAIT

RORB

NR1F2, ROR-BETA, RZR-BETA, RZRB, bA133M9.1

RAR-related orphan receptor B

Autism spectrum disorder (ASD) Project

GO Process (14)

GO Function (2)

GO Component (2)

Gene Ontology Molecular Function

protein binding [IPI]
sequence-specific DNA binding transcription factor activity [ISS]

Gene Ontology Cellular Component

nucleoplasm [TAS]

CRISPR Database OMIM HGNC Alliance of Genome Resources Entrez Gene RefSeq UniprotKB Ensembl

Homo sapiens

PREY

UBE2I

C358B7.1, P18, UBC9, LA16c-358B7.1

ubiquitin-conjugating enzyme E2I

GO Process (8)

GO Function (9)

GO Component (6)

Gene Ontology Biological Process

cellular protein metabolic process [TAS]

cellular protein modification process [TAS]

negative regulation of transcription from RNA polymerase II promoter [IMP]

negative regulation of transcription, DNA-templated [IDA]

post-translational protein modification [TAS]

protein sumoylation [IDA, TAS]

protein ubiquitination [IBA]

ubiquitin-dependent protein catabolic process [TAS]

Gene Ontology Molecular Function

RING-like zinc finger domain binding [IPI]
SUMO transferase activity [IDA]
enzyme binding [IPI]
poly(A) RNA binding [IDA]
protein binding [IPI]
transcription factor binding [IPI]
ubiquitin protein ligase activity [IBA]
ubiquitin protein ligase binding [IBA]
ubiquitin-protein transferase activity [TAS]

Gene Ontology Cellular Component

cytoplasm [IDA]

nucleoplasm [TAS]

synaptonemal complex [TAS]

transferase complex [IDA]

CRISPR Database OMIM HGNC Alliance of Genome Resources Entrez Gene RefSeq UniprotKB Ensembl

Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Automated yeast two-hybrid screening for nuclear receptor-interacting proteins.

Albers M, Kranz H, Kober I, Kaiser C, Klink M, Suckow J, Kern R, Koegl M

High throughput analysis of protein-protein interactions is an important sector of hypothesis-generating research. Using an improved and automated version of the yeast two-hybrid system, we completed a large interaction screening project with a focus on nuclear receptors and their cofactors. A total of 425 independent yeast two-hybrid cDNA library screens resulted in 6425 potential interacting protein fragments involved in 1613 ... [more]

Mol. Cell Proteomics Feb. 01, 2005; 4(2);205-13 [Pubmed: 15604093]

Throughput

Low Throughput

Curated By

BioGRID