BAIT
NR1H4
BAR, FXR, HRR-1, HRR1, RIP14
nuclear receptor subfamily 1, group H, member 4
GO Process (15)
GO Function (13)
GO Component (2)
Gene Ontology Biological Process
- cellular response to bile acid [ISS]
- cellular response to organonitrogen compound [ISS]
- gene expression [TAS]
- intracellular bile acid receptor signaling pathway [IDA]
- intracellular receptor signaling pathway [ISS]
- negative regulation of bile acid biosynthetic process [IDA]
- negative regulation of transcription from RNA polymerase II promoter [ISS]
- nitrogen catabolite activation of transcription from RNA polymerase II promoter [IC]
- positive regulation of glutamate metabolic process [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of bile acid biosynthetic process [TAS]
- regulation of cholesterol metabolic process [TAS]
- regulation of urea metabolic process [ISS]
- signal transduction [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
Gene Ontology Molecular Function- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [IDA]
- bile acid binding [ISS, TAS]
- bile acid receptor activity [IDA]
- chenodeoxycholic acid binding [IDA]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [ISS]
- ligand-dependent nuclear receptor binding [TAS]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [TAS]
- transcription coactivator activity [TAS]
- transcription corepressor activity [TAS]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [IDA]
- bile acid binding [ISS, TAS]
- bile acid receptor activity [IDA]
- chenodeoxycholic acid binding [IDA]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [ISS]
- ligand-dependent nuclear receptor binding [TAS]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [TAS]
- transcription coactivator activity [TAS]
- transcription corepressor activity [TAS]
Gene Ontology Cellular Component
Homo sapiens
PREY
GAPDH
G3PD, GAPD, HEL-S-162eP, CDABP0047
glyceraldehyde-3-phosphate dehydrogenase
GO Process (11)
GO Function (5)
GO Component (13)
Gene Ontology Biological Process
- carbohydrate metabolic process [TAS]
- cellular response to interferon-gamma [IDA]
- gluconeogenesis [TAS]
- glucose metabolic process [TAS]
- glycolytic process [TAS]
- microtubule cytoskeleton organization [ISS]
- negative regulation of translation [IDA, IMP]
- neuron apoptotic process [ISS]
- peptidyl-cysteine S-trans-nitrosylation [ISS]
- protein stabilization [ISS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- GAIT complex [IDA]
- cytoplasm [IDA, ISS]
- cytosol [IDA, ISS, TAS]
- extracellular vesicular exosome [IDA]
- intracellular membrane-bounded organelle [IDA]
- lipid particle [IDA]
- membrane [IDA]
- microtubule cytoskeleton [ISS]
- nuclear membrane [IDA]
- nucleus [IDA, ISS]
- plasma membrane [IDA]
- ribonucleoprotein complex [IDA]
- vesicle [IDA]
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Automated yeast two-hybrid screening for nuclear receptor-interacting proteins.
High throughput analysis of protein-protein interactions is an important sector of hypothesis-generating research. Using an improved and automated version of the yeast two-hybrid system, we completed a large interaction screening project with a focus on nuclear receptors and their cofactors. A total of 425 independent yeast two-hybrid cDNA library screens resulted in 6425 potential interacting protein fragments involved in 1613 ... [more]
Mol. Cell Proteomics Feb. 01, 2005; 4(2);205-13 [Pubmed: 15604093]
Throughput
- Low Throughput
Curated By
- BioGRID