Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Automated yeast two-hybrid screening for nuclear receptor-interacting proteins.

Albers M, Kranz H, Kober I, Kaiser C, Klink M, Suckow J, Kern R, Koegl M

High throughput analysis of protein-protein interactions is an important sector of hypothesis-generating research. Using an improved and automated version of the yeast two-hybrid system, we completed a large interaction screening project with a focus on nuclear receptors and their cofactors. A total of 425 independent yeast two-hybrid cDNA library screens resulted in 6425 potential interacting protein fragments involved in 1613 ... [more]

Mol. Cell Proteomics Feb. 01, 2005; 4(2);205-13 [Pubmed: 15604093]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

HNF4G

Gene Ontology Biological Process

Gene Ontology Molecular Function

sequence-specific DNA binding transcription factor activity [TAS]

Gene Ontology Cellular Component

PRDX6