BAIT

HAC1

ERN4, IRE15, transcription factor HAC1, L000002611, YFL031W
Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress
Saccharomyces cerevisiae (S288c)
PREY

LCL2

YLR104W
Putative protein of unknown function; mutant is deficient in amounts of cell wall mannosylphosphate and has long chronological lifespan; genetic interactions suggest a role in ER-associated protein degradation (ERAD)
GO Process (1)
GO Function (0)
GO Component (0)

Gene Ontology Biological Process

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Secretory pathway genes assessed by high-throughput microscopy and synthetic genetic array analysis.

Bircham PW, Maass DR, Roberts CA, Kiew PY, Low YS, Yegambaram M, Matthews J, Jack CA, Atkinson PH

We developed a procedure for automated confocal microscopy to image the effect of the non-essential yeast gene deletion set on the localisation of the plasma membrane GFP-labelled protein Mrh1p-GFP. To achieve this it was necessary to devise an expression system expressing Redstar2 RFP-fluorescence specifically in the nucleus, mCherry RFP at a lower intensity in the cytoplasm and Mrh1p-GFP in the ... [more]

Mol Biosyst Sep. 01, 2011; 7(9);2589-98 [Pubmed: 21731954]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)
  • phenotype: colony size (APO:0000063)

Additional Notes

  • High Throughput: Synthetic Genetic Array (SGA) analysis

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HAC1 LCL2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1724BioGRID
377311
LCL2 HAC1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-5.9636BioGRID
581496

Curated By

  • BioGRID