BAIT

HAC1

ERN4, IRE15, transcription factor HAC1, L000002611, YFL031W
Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress
Saccharomyces cerevisiae (S288c)
PREY

CWH41

DER7, GLS1, L000003121, L000000715, YGL027C
Processing alpha glucosidase I; ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress
GO Process (2)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Secretory pathway genes assessed by high-throughput microscopy and synthetic genetic array analysis.

Bircham PW, Maass DR, Roberts CA, Kiew PY, Low YS, Yegambaram M, Matthews J, Jack CA, Atkinson PH

We developed a procedure for automated confocal microscopy to image the effect of the non-essential yeast gene deletion set on the localisation of the plasma membrane GFP-labelled protein Mrh1p-GFP. To achieve this it was necessary to devise an expression system expressing Redstar2 RFP-fluorescence specifically in the nucleus, mCherry RFP at a lower intensity in the cytoplasm and Mrh1p-GFP in the ... [more]

Mol Biosyst Sep. 01, 2011; 7(9);2589-98 [Pubmed: 21731954]

Throughput

  • High Throughput

Ontology Terms

  • vegetative growth (APO:0000106)
  • colony size (APO:0000063)
  • resistance to chemicals (APO:0000087)

Additional Notes

  • High Throughput: Synthetic Genetic Array (SGA) analysis was used to identify gene deletions causing hypersensitivity to DTT in the absence HAC1.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CWH41 HAC1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-5.8426BioGRID
898444

Curated By

  • BioGRID