BAIT

TPR

2610029M07Rik, C77892

translocated promoter region, nuclear basket protein

GO Process (18)

GO Function (10)

GO Component (11)

Gene Ontology Biological Process

MAPK import into nucleus [ISO]

RNA export from nucleus [ISO]

RNA import into nucleus [ISO]

cellular response to heat [ISO]

cellular response to interferon-alpha [IDA]

mRNA export from nucleus in response to heat stress [ISO]

mitotic spindle assembly checkpoint [ISO]

negative regulation of RNA export from nucleus [ISO]

negative regulation of transcription from RNA polymerase II promoter [ISO]

negative regulation of translational initiation [ISO]

positive regulation of heterochromatin assembly [ISO]

positive regulation of intracellular protein transport [ISO]

positive regulation of mitotic cell cycle spindle assembly checkpoint [ISO]

positive regulation of protein export from nucleus [ISO]

positive regulation of protein import into nucleus [ISO]

regulation of mitotic sister chromatid separation [ISO]

regulation of spindle assembly involved in mitosis [ISO]

response to epidermal growth factor [ISO]

Gene Ontology Molecular Function

chromatin binding [ISO]
dynein complex binding [ISO]
heat shock protein binding [ISO]
mRNA binding [ISO]
mitogen-activated protein kinase binding [ISO]
nucleocytoplasmic transporter activity [ISO]
poly(A) RNA binding [ISO]
protein binding [IPI]
protein homodimerization activity [ISO]
tubulin binding [ISO]

Gene Ontology Cellular Component

cytoplasm [ISO]

cytoplasmic dynein complex [ISO]

extrinsic component of membrane [ISO]

mitotic spindle [ISO]

nuclear envelope [IDA, ISO]

nuclear inclusion body [IDA, ISO]

nuclear membrane [ISO]

nuclear periphery [IDA, ISO]

nuclear pore [IDA, ISO]

nuclear pore nuclear basket [ISO]

CRISPR Database MGI Alliance of Genome Resources VEGA Entrez Gene RefSeq UniprotKB Ensembl

Mus musculus

PREY

PPIA

CYPA, CYPH, HEL-S-69p

peptidylprolyl isomerase A (cyclophilin A)

GO Process (18)

GO Function (5)

GO Component (7)

Gene Ontology Molecular Function

peptidyl-prolyl cis-trans isomerase activity [IDA]
poly(A) RNA binding [IDA]
protein binding [IPI]
unfolded protein binding [TAS]
virion binding [NAS]

Gene Ontology Cellular Component

cytosol [IDA, TAS]

extracellular region [TAS]

extracellular space [IDA]

extracellular vesicular exosome [IDA]

focal adhesion [IDA]

CRISPR Database VEGA HGNC Alliance of Genome Resources OMIM Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Systematic analysis of human protein complexes identifies chromosome segregation proteins.

Hutchins JR, Toyoda Y, Hegemann B, Poser I, Heriche JK, Sykora MM, Augsburg M, Hudecz O, Buschhorn BA, Bulkescher J, Conrad C, Comartin D, Schleiffer A, Sarov M, Pozniakovsky A, Slabicki MM, Schloissnig S, Steinmacher I, Leuschner M, Ssykor A, Lawo S, Pelletier L, Stark H, Nasmyth K, Ellenberg J, Durbin R, Buchholz F, Mechtler K, Hyman AA, Peters JM

Chromosome segregation and cell division are essential, highly ordered processes that depend on numerous protein complexes. Results from recent RNA interference screens indicate that the identity and composition of these protein complexes is incompletely understood. Using gene tagging on bacterial artificial chromosomes, protein localization, and tandem-affinity purification-mass spectrometry, the MitoCheck consortium has analyzed about 100 human protein complexes, many of ... [more]

Science Apr. 30, 2010; 328(5978);593-9 [Pubmed: 20360068]

Throughput

High Throughput

Curated By

BioGRID