BAIT
PLK1
Plk, STPK13
polo-like kinase 1
GO Process (27)
GO Function (6)
GO Component (12)
Gene Ontology Biological Process
- G2 DNA damage checkpoint [ISO]
- G2/M transition of mitotic cell cycle [ISO]
- activation of mitotic anaphase-promoting complex activity [ISO]
- centrosome organization [ISO]
- cytokinesis [ISO]
- establishment of protein localization [ISO]
- microtubule bundle formation [ISO]
- mitotic cytokinesis [ISO]
- mitotic nuclear division [ISO]
- mitotic sister chromatid segregation [ISO]
- mitotic spindle assembly checkpoint [ISO]
- negative regulation of apoptotic process [ISO]
- negative regulation of cyclin-dependent protein serine/threonine kinase activity [ISO]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- peptidyl-serine phosphorylation [ISO]
- polar body extrusion after meiotic divisions [ISO]
- positive regulation of peptidyl-threonine phosphorylation [ISO]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [ISO]
- positive regulation of proteolysis [ISO]
- positive regulation of ubiquitin-protein transferase activity [ISO]
- protein destabilization [ISO]
- protein localization to chromatin [ISO]
- protein phosphorylation [IDA, ISO]
- protein ubiquitination [ISO]
- regulation of mitotic cell cycle [ISO]
- regulation of mitotic metaphase/anaphase transition [ISO]
- regulation of protein binding [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
STXBP4
Synip
syntaxin binding protein 4
GO Process (5)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Systematic analysis of human protein complexes identifies chromosome segregation proteins.
Chromosome segregation and cell division are essential, highly ordered processes that depend on numerous protein complexes. Results from recent RNA interference screens indicate that the identity and composition of these protein complexes is incompletely understood. Using gene tagging on bacterial artificial chromosomes, protein localization, and tandem-affinity purification-mass spectrometry, the MitoCheck consortium has analyzed about 100 human protein complexes, many of ... [more]
Science Apr. 30, 2010; 328(5978);593-9 [Pubmed: 20360068]
Throughput
- High Throughput
Curated By
- BioGRID