BAIT
VPS30
APG6, ATG6, VPT30, L000003545, YPL120W
Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; C-terminus has a novel globular fold that is essential for autophagy through the targeting of the PI3-kinase complex I to the pre-autophagosomal structure; ortholog of the higher eukaryotic gene Beclin 1
GO Process (8)
GO Function (0)
GO Component (5)
Gene Ontology Biological Process
Saccharomyces cerevisiae (S288c)
PREY
PIM1
ATP-dependent Lon protease PIM1, LON1, L000000949, L000001440, YBL022C
ATP-dependent Lon protease; involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria
GO Process (7)
GO Function (4)
GO Component (3)
Gene Ontology Biological Process
- cellular response to oxidative stress [IBA]
- chaperone-mediated protein complex assembly [IGI]
- misfolded or incompletely synthesized protein catabolic process [IGI, IMP]
- mitochondrion organization [IBA]
- oxidation-dependent protein catabolic process [IBA]
- protein homooligomerization [IBA]
- response to hypoxia [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
A mitochondrial-focused genetic interaction map reveals a scaffold-like complex required for inner membrane organization in mitochondria.
To broadly explore mitochondrial structure and function as well as the communication of mitochondria with other cellular pathways, we constructed a quantitative, high-density genetic interaction map (the MITO-MAP) in Saccharomyces cerevisiae. The MITO-MAP provides a comprehensive view of mitochondrial function including insights into the activity of uncharacterized mitochondrial proteins and the functional connection between mitochondria and the ER. The MITO-MAP ... [more]
J. Cell Biol. Oct. 17, 2011; 195(2);323-40 [Pubmed: 21987634]
Quantitative Score
- -4.385553211 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- An Epistatic MiniArray Profile (E-MAP) approach was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score >= 2.0 for positive interactions (epistatic or suppressor interactions) and S score <= -2.5 for negative interactions (synthetic sick/lethal interactions). The authors constructed a mitochondrial-focused genetic interaction map (the MITO-MAP).
Curated By
- BioGRID