BAIT
IRA2
CCS1, GLC4, Ras GTPase activating protein IRA2, L000000874, YOL081W
GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; IRA2 has a paralog, IRA1, that arose from the whole genome duplication; defects in human homolog NF1 are associated with neurofibromatosis
GO Process (7)
GO Function (1)
GO Component (5)
Gene Ontology Biological Process
- cellular response to cold [IMP]
- cellular response to hydrogen peroxide [IMP]
- cellular response to metal ion [IMP]
- cellular response to oxidative stress [IMP]
- negative regulation of Ras protein signal transduction [IMP]
- negative regulation of cAMP biosynthetic process [IMP]
- positive regulation of Ras GTPase activity [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
CYS4
NHS5, STR4, VMA41, cystathionine beta-synthase CYS4, L000001248, L000000471, YGR155W
Cystathionine beta-synthase; catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog CBS cause homocystinuria
GO Process (5)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
A mitochondrial-focused genetic interaction map reveals a scaffold-like complex required for inner membrane organization in mitochondria.
To broadly explore mitochondrial structure and function as well as the communication of mitochondria with other cellular pathways, we constructed a quantitative, high-density genetic interaction map (the MITO-MAP) in Saccharomyces cerevisiae. The MITO-MAP provides a comprehensive view of mitochondrial function including insights into the activity of uncharacterized mitochondrial proteins and the functional connection between mitochondria and the ER. The MITO-MAP ... [more]
J. Cell Biol. Oct. 17, 2011; 195(2);323-40 [Pubmed: 21987634]
Quantitative Score
- -3.575425626 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- An Epistatic MiniArray Profile (E-MAP) approach was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score >= 2.0 for positive interactions (epistatic or suppressor interactions) and S score <= -2.5 for negative interactions (synthetic sick/lethal interactions). The authors constructed a mitochondrial-focused genetic interaction map (the MITO-MAP).
Curated By
- BioGRID