BAIT

PPARGC1A

LEM6, PGC-1(alpha), PGC-1v, PGC1, PGC1A, PPARGC1
peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
GO Process (32)
GO Function (12)
GO Component (3)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

RNF34 is a cold-regulated E3 ubiquitin ligase for PGC-1α and modulates brown fat cell metabolism.

Wei P, Pan D, Mao C, Wang YX

The transcriptional co-activator PGC-1α is a master regulator of energy metabolism and adaptive thermogenesis in the brown fat. PGC-1α is a short-lived protein, but the molecular components that control PGC-1α turnover and their functional importance in energy metabolism are largely unknown. Here we perform a luciferase-based overexpression screen and identify a Ring-finger-containing protein, RNF34, as a specific E3 ubiquitin ligase ... [more]

Unknown Nov. 07, 2011; 0(0); [Pubmed: 22064484]

Throughput

  • Low Throughput

Curated By

  • BioGRID