BAIT

CIN8

KSL2, SDS15, kinesin motor protein CIN8, L000000340, YEL061C
Kinesin motor protein; involved in mitotic spindle assembly and chromosome segregation
Saccharomyces cerevisiae (S288c)
PREY

CIN8

KSL2, SDS15, kinesin motor protein CIN8, L000000340, YEL061C
Kinesin motor protein; involved in mitotic spindle assembly and chromosome segregation
Saccharomyces cerevisiae (S288c)

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Directionality of individual kinesin-5 Cin8 motors is modulated by loop 8, ionic strength and microtubule geometry.

Gerson-Gurwitz A, Thiede C, Movshovich N, Fridman V, Podolskaya M, Danieli T, Lakaemper S, Klopfenstein DR, Schmidt CF, Gheber L

Kinesin-5 motors fulfil essential roles in mitotic spindle morphogenesis and dynamics as slow, processive microtubule (MT) plus-end directed motors. The Saccharomyces cerevisiae kinesin-5 Cin8 was found, surprisingly, to switch directionality. Here, we have examined directionality using single-molecule fluorescence motility assays and live-cell microscopy. On spindles, Cin8 motors mostly moved slowly (∼25 nm/s) towards the midzone, but occasionally also faster (∼55 ... [more]

Unknown Nov. 18, 2011; 0(0); [Pubmed: 22101328]

Throughput

  • Low Throughput

Additional Notes

  • Cin8 forms homotetramer

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CIN8 CIN8
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
CIN8 CIN8
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-
CIN8 CIN8
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By

  • BioGRID