An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Kinase suppressor of Ras forms a multiprotein signaling complex and modulates MEK localization.

Stewart S, Sundaram M, Zhang Y, Lee J, Han M, Guan KL

Genetic screens for modifiers of activated Ras phenotypes have identified a novel protein, kinase suppressor of Ras (KSR), which shares significant sequence homology with Raf family protein kinases. Studies using Drosophila melanogaster and Caenorhabditis elegans predict that KSR positively regulates Ras signaling; however, the function of mammalian KSR is not well understood. We show here that two predicted kinase-dead mutants ... [more]

Mol. Cell. Biol. Aug. 01, 1999; 19(8);5523-34 [Pubmed: 10409742]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
KSR1 HSPA4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Zhou L (2015)	High	-	BioGRID	1512830

Curated By

BioGRID

Interaction Summary

KSR1

Gene Ontology Biological Process

Gene Ontology Molecular Function

MAP-kinase scaffold activity [IDA]mitogen-activated protein kinase kinase binding [ISO]protein C-terminus binding [ISO]protein binding [IPI]

Gene Ontology Cellular Component

HSPA4