BAIT
INSIG2
insulin induced gene 2
GO Process (2)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ENO1
ENO1L1, MPB1, NNE, PPH
enolase 1, (alpha)
GO Process (9)
GO Function (4)
GO Component (6)
Gene Ontology Biological Process
- carbohydrate metabolic process [TAS]
- gluconeogenesis [TAS]
- glucose metabolic process [TAS]
- glycolytic process [TAS]
- negative regulation of cell growth [IDA]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- negative regulation of transcription, DNA-templated [IDA]
- response to virus [IEP]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Regulated endoplasmic reticulum-associated degradation of a polytopic protein: p97 recruits proteasomes to Insig-1 before extraction from membranes.
Polytopic membrane proteins subjected to endoplasmic reticulum (ER)-associated degradation are extracted from membranes and targeted to proteasomes for destruction. The extraction mechanism is poorly understood. One polytopic ER protein subjected to ER-associated degradation is Insig-1, a negative regulator of cholesterol synthesis. Insig-1 is rapidly degraded by proteasomes when cells are depleted of cholesterol, and its degradation is inhibited when sterols ... [more]
J. Biol. Chem. Dec. 11, 2009; 284(50);34889-900 [Pubmed: 19815544]
Throughput
- High Throughput
Curated By
- BioGRID