BAIT

SCS2

phosphatidylinositol-binding protein SCS2, L000002629, YER120W
Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)
PREY

SMT3

SUMO family protein SMT3, L000001938, YDR510W
Ubiquitin-like protein of the SUMO family; conjugated to lysine residues of target proteins; associates with transcriptionally active genes; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2
GO Process (1)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

Desumoylation of the ER Membrane VAP Protein Scs2 by Ulp1 and SUMO Regulation of the Inositol Synthesis Pathway.

Felberbaum R, Wilson NR, Cheng D, Peng J, Hochstrasser M

Post-translational protein modification by the ubiquitin-like SUMO protein is critical to eukaryotic cell regulation, but much remains unknown regarding its operation and substrates. Here we report that specific mutations in the Saccharomyces cerevisiae Ulp1 SUMO protease, including its coiled-coil (CC) domain, lead to the accumulation of distinct sumoylated proteins in vivo. A prominent ∼50 kDa sumoylated protein accumulates in a ... [more]

Unknown Oct. 24, 2011; 0(0); [Pubmed: 22025676]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: auxotrophy (APO:0000097)

Additional Notes

  • CEN expression of SMT3GG partially suppresses inositol auxotrophy of ulp2 scs2

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SCS2 SMT3
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1377BioGRID
2040640

Curated By

  • BioGRID