BAIT

ARRB1

ARB1, ARR1
arrestin, beta 1
GO Process (22)
GO Function (9)
GO Component (11)
Homo sapiens
PREY

STUB1

CHIP, HSPABP2, NY-CO-7, SCAR16, SDCCAG7, UBOX1, LA16c-313D11.6
STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase
GO Process (15)
GO Function (15)
GO Component (10)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

beta-Arrestins facilitate ubiquitin-dependent degradation of apoptosis signal-regulating kinase 1 (ASK1) and attenuate H2O2-induced apoptosis.

Zhang Z, Hao J, Zhao Z, Ben P, Fang F, Shi L, Gao Y, Liu J, Wen C, Luo L, Yin Z

beta-Arrestins are ubiquitously expressed proteins that play important roles in receptor desensitization, endocytosis, proteosomal degradation, apoptosis and signaling. It has been reported that beta-Arrestin2 acts as a scaffold by directly interacting with the JNK3 isoform and recruiting MKK4 and the apoptosis-signaling kinase-1 (ASK1). Here, we report a novel function of beta-Arrestins in regulating H(2)O(2)-induced apoptosis. Our study demonstrates that beta-Arrestins ... [more]

Cell. Signal. Jul. 01, 2009; 21(7);1195-206 [Pubmed: 19306926]

Throughput

  • Low Throughput

Curated By

  • BioGRID