An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

BAG3 mediates chaperone-based aggresome-targeting and selective autophagy of misfolded proteins.

Gamerdinger M, Kaya AM, Wolfrum U, Clement AM, Behl C

Increasing evidence indicates the existence of selective autophagy pathways, but the manner in which substrates are recognized and targeted to the autophagy system is poorly understood. One strategy is transport of a particular substrate to the aggresome, a perinuclear compartment with high autophagic activity. In this paper, we identify a new cellular pathway that uses the specificity of heat-shock protein ... [more]

EMBO Rep. Feb. 01, 2011; 12(2);149-56 [Pubmed: 21252941]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
DYNLT1 BAG3	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Gamerdinger M (2011)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

DYNLT1

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

BAG3