PIN1
Gene Ontology Biological Process
- negative regulation of ERK1 and ERK2 cascade [ISO]
- negative regulation of cell motility [ISO]
- negative regulation of neuron apoptotic process [ISO]
- negative regulation of transforming growth factor beta receptor signaling pathway [ISO]
- positive regulation of Rho GTPase activity [ISO]
- positive regulation of cell growth involved in cardiac muscle cell development [ISO]
- positive regulation of neuron apoptotic process [ISO]
- positive regulation of protein dephosphorylation [ISO]
- positive regulation of protein phosphorylation [ISO]
- positive regulation of ubiquitin-protein transferase activity [ISO]
- protein peptidyl-prolyl isomerization [ISO]
- regulation of cell proliferation [IMP]
- regulation of cytokinesis [IMP, ISO]
- regulation of pathway-restricted SMAD protein phosphorylation [ISO]
Gene Ontology Molecular Function
NANOG
Gene Ontology Biological Process
- cell proliferation [ISO]
- embryo development [IMP]
- endodermal cell fate specification [ISO]
- mesodermal cell fate commitment [IGI]
- negative regulation of BMP signaling pathway [IDA]
- negative regulation of cell differentiation [IDA]
- negative regulation of cell fate commitment [IDA, IGI]
- negative regulation of endodermal cell fate specification [TAS]
- negative regulation of transcription from RNA polymerase II promoter [IGI, IMP, ISO]
- positive regulation of cell proliferation [IDA]
- positive regulation of mitotic cell cycle [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IMP, ISO]
- positive regulation of transcription, DNA-templated [IMP]
- regulation of cell differentiation [ISO]
- regulation of gene expression [ISO]
- regulation of transcription, DNA-templated [ISO]
- response to organic substance [IDA]
- response to retinoic acid [IDA]
- somatic stem cell maintenance [ISO]
- stem cell differentiation [IDA, IMP]
- stem cell division [IDA]
- stem cell maintenance [IDA, IMP]
Gene Ontology Molecular Function- DNA binding [IDA, ISO]
- chromatin binding [IDA]
- enhancer sequence-specific DNA binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IMP, ISO]
- transcription corepressor activity [IMP]
- transcription regulatory region DNA binding [IDA]
- DNA binding [IDA, ISO]
- chromatin binding [IDA]
- enhancer sequence-specific DNA binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IMP, ISO]
- transcription corepressor activity [IMP]
- transcription regulatory region DNA binding [IDA]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Phosphorylation stabilizes Nanog by promoting its interaction with Pin1.
Embryonic stem cells (ESCs) can undergo unlimited self-renewal and retain the pluripotency to differentiate into all cell types in the body, thus holding great promise as a renewable source of cells for human therapy. The mechanisms that maintain self-renewal of ESCs remain unclear. Here we show that Nanog, a transcription factor crucial for the self-renewal of ESCs, is phosphorylated at ... [more]
Throughput
- Low Throughput
Additional Notes
- source of Nanog not clear
- source of Pin1 not clear
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NANOG PIN1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 590939 | |
| PIN1 NANOG | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 590938 |
Curated By
- BioGRID