BAIT

MDM2

ACTFS, HDMX, hdm2
MDM2 proto-oncogene, E3 ubiquitin protein ligase
GO Process (25)
GO Function (7)
GO Component (9)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Trp53 regulates Notch 4 signaling through Mdm2.

Sun Y, Klauzinska M, Lake RJ, Lee JM, Santopietro S, Raafat A, Salomon D, Callahan R, Artavanis-Tsakonas S

Notch receptors and their ligands have crucial roles in development and tumorigenesis. We present evidence demonstrating the existence of an antagonistic relationship between Notch 4 and Trp53, which is controlled by the Mdm2-dependent ubiquitylation and degradation of the Notch receptor. We show that this signal-controlling mechanism is mediated by physical interactions between Mdm2 and Notch 4 and suggest the existence ... [more]

J. Cell. Sci. Apr. 01, 2011; 124(0);1067-76 [Pubmed: 21402876]

Throughput

  • Low Throughput

Curated By

  • BioGRID