BAIT

NFE2L1

LCR-F1, NRF1, TCF11
nuclear factor, erythroid 2-like 1
GO Process (4)
GO Function (1)
GO Component (0)
Homo sapiens
PREY

FBXW7

AGO, CDC4, FBW6, FBW7, FBX30, FBXO30, FBXW6, SEL-10, SEL10, hAgo, hCdc4
F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase
GO Process (24)
GO Function (6)
GO Component (6)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The Fbw7 Tumor Suppressor Regulates Nuclear Factor E2-related Factor 1 Transcription Factor Turnover through Proteasome-mediated Proteolysis.

Biswas M, Phan D, Watanabe M, Chan JY

Nuclear factor E2-related factor 1 (Nrf1) is a basic leucine zipper transcription factor that plays important roles in cellular stress response and development. Currently, the mechanism regulating Nrf1 expression is poorly understood. We report here that Nrf1 is a short-lived protein that is targeted by F-box protein Fbw7, which is the substrate-specifying component of SCF (Skp1-Cul1-Fbox protein-Rbx1)-type ubiquitin ligase for ... [more]

J. Biol. Chem. Nov. 11, 2011; 286(45);39282-9 [Pubmed: 21953459]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NFE2L1 FBXW7
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
858761

Curated By

  • BioGRID